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. 2017 Jul 19;8:1272. doi: 10.3389/fpls.2017.01272

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Copyright © 2017 Nelson and Oliver.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Arrangement of seedlings in soil-plates. (A) Seedlings are sterilized and plated into soil-plates in staggered rows of five. A 16 mm plastic strip is inserted into the soil to serve as a scale during analysis of digital photographs. Seedlings are germinated vertically and transplanted at 30 h by selecting only seedlings at the same developmental stage. (B) Representation of the plate side view containing a single seedling. Seed angle is important for wheat during planting so that roots and shoot emerge at the same plane as the surface of the soil and the transparent plate lid.