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Cultured HSCs differentiate towards neurons and glia cells in vitro. Cultured HSCs differentiated toward neurons (A–C) and astrocytes (D–F), which were identified by immunofluorecent staining of Tuj1 and aldehyde dehydrogenase 1 family, member L1 (ALDH1L1) antibodies, respectively, while the negative control showed no positive staining (G–I). Bar = 25 μm. White arrows represented the positive cells.
