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. 2017 Jul 19;11:213. doi: 10.3389/fncel.2017.00213

Figure 4.

Figure 4

Transplanted HSCs could differentiate into neurons and glia like cells in vivo. (A) Positive HSCs with blue staining in nucleus marked by Hoechst 33342 in the spinal cord could be seen after HSC transplantation. (B) A few of HSCs could differentiate into neurons with Tuj1 positive staining (red). (C) The merged picture of Tuj1 and Hoechst 33342, the positive ratio of Tuj1/ Hoechst 33342 is about 3.1%. (D) Positive HSCs with Hoechst 33342 blue staining. (E) A few of HSCs could differentiate into astrocytes with ALDH1L1 positive staining (red). (F) The merged picture of ALDH1L1 and Hoechst 33342, which showed the positive ratio is about 18.3%. (G) Hoechst 33342 blue staining in the negative control (no primary antibody). (H) There is no positive red staining in the negative control (no primary antibody). (I) The merged picture. Bar = 50 μm. White arrows represented the positive cells. The positive ratio of Tuj1 (Tuj1/ Hoechst 33342) and ALDH1L1 (ALDH1L1/Hoechst 33342) was quantified using Image-Pro Plus 6.0 software (n = 7).