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. 2017 Jul 18;7:5781. doi: 10.1038/s41598-017-06173-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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GSK-3β deletion in DG decreases several synapse-associated proteins. (a,b) GSK-3β deletion decreased the levels of multiple pre- and post-synaptic proteins in DG extracts measured by Western blotting and the quantitative analyses (n = 4 each group) (* by unpaired t test, # by multiple t-tests using Sidak-Bonferroin method of GraphPad Prism 6.0). (c,d) GSK-3β deletion in DG excitatory neurons did not change the synapse-associated protein in prefrontal cortex (n = 3 each group) (* by unpaired t test, ^# by multiple t-tests using Sidak-Bonferroin method of GraphPad Prism 6.0). (e,f) The representative images of the hippocampus by Nissl staining and the densitometric analyses (n = 5–6 each group, unpaired t test). Scale bars, 100 μm. Data were presented as mean ± s.e.m. *P < 0.05, **P < 0.01,***P < 0.001 versus Vector. “#” indicates that the difference is significant analyzed by multiple t-tests. The absence of asterix indicates that the difference is not significant.