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. 2017 Jul 18;7:5781. doi: 10.1038/s41598-017-06173-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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GSK-3β deletion inhibits CaMKII/IV-CREB signaling. (a,b) GSK-3β deletion decreased total and/or the phosphorylated levels of CaMKII, CaMKIV and CREB in DG extracts measured by Western blotting and the quantitative analyses. (c) The reduced intensity of pCREB in DG excitatory neurons was also shown by co-immunofluorescent staining. Scale bars, 100 μm. Data were presented as mean ± s.e.m. n = 4 each group, analyzed by unpaired t-test (*) or multiple t-tests (#). *P < 0.05, **P < 0.01, *** < 0.001 versus Vector, “#” indicates that the difference is significant analyzed by multiple t-tests. the absence of asterix indicates that the difference is not significant.