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. 2017 Jul 18;7:5658. doi: 10.1038/s41598-017-05978-7

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Ambient preservation of neutrophils. (A) Percent migration was established for isolated neutrophils following ambient storage (0 h v 72 h control, p < 0.01, t-test). (B) The migratory speed further shows that Q-VD-OPh treated cells appear indistinguishable from fresh neutrophils (0 h v 72 h control, p < 0.0001, t-test). (C) Following storage, the percentage of neutrophils that undergo NETosis over time in response to 10 nM PMA was quantified. Q-VD-OPh treated cells form more NETs compared to the untreated control (p < 0.001, Two-way ANOVA with Bonferroni multiple comparison post-test). The ability for Q-VD-OPh treated neutrophils to form NETs is not significantly impacted following 72 h ambient storage. (D) NETs formation after 240 min PMA stimulation in DAPI-stained neutrophils following 72–96 h ambient storage. All error bars represent standard deviation, n = 3.