Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Jul 18;7:5691. doi: 10.1038/s41598-017-05932-7

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

TRAIL regulates the production of cytokines and chemokines in a time- and dose-dependent manner. (A–D) SGBS adipocytes on day 14 of adipogenic differentiation were treated with TRAIL (30 ng/ml) or vehicle for 6, 12 and 24 hours. The mRNA expression of IL-6 (A), IL-8 (B), MCP-1 (C) and CCL-20 (D) was analyzed by qPCR. The mRNA levels were normalized to HPRT. Depicted are the means and SEM of 4 independent experiments. Two-way ANOVA and Sidak’s multiple comparison were used to test for statistical significance. (E–H) SGBS adipocytes on day 14 of adipogenic differentiation were treated with increasing doses of TRAIL or vehicle for 6 hours. The mRNA expression of IL-6 (E), IL-8 (F), MCP-1 (G) and CCL-20 (H) was analyzed by qPCR. The mRNA levels were normalized to HPRT. Depicted are the means and SEM of 3 independent experiments. One-way ANOVA and Dunnett’s multiple comparison were used to test for statistical significance. (I–L) SGBS adipocytes on day 14 of adipogenic differentiation were treated with TRAIL (30 ng/ml) or vehicle. After 24 hours, media supernatants were collected and IL-6 (I), IL-8 (J), MCP-1 (K) and CCL-20 (L) concentrations were determined by ELISA. Means and SEM of 3–7 independent experiments are shown. Unpaired Student’s t-tests were used to test for statistical significance. *p < 0.05, **p < 0.01, ***p < 0.001.