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. 2017 Jul 18;7:5704. doi: 10.1038/s41598-017-05571-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Measurements of mtDNA copy numbers in lymphoblastoid cell lines. The mtDNA copy numbers were determined by comparing the ratio of mtDNA to nDNA (β-actin) by real-time quantitative PCR. (a) The ratio of MT-D-loop region to β-actin. (b) The ratio of MT-ND1 to β-actin. The calculations were based on three independent determinations. The error bars indicate two standard errors of the mean (SEM). P indicates the significance, according to the Student t-test, of the differences between the mean of mutant and that of control cell lines.