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. 2017 Jul 18;7:5650. doi: 10.1038/s41598-017-05995-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Characterization of p53 in the AG flies. (a) Co-immunoprecipitation of Hsp26/Hsp27 with p53. The homogenized fly heads were immunoprecipitated with anti-Hsp26 or anti-Hsp27 antibodies and probed with anti-Hsp26, anti-Hsp27, anti-p53 antibody, with anti-IgG as a control. The input of actin is the protein loading control. The genotypes of flies are indicated on the image. Trial n = 2. (b) Accumulation of p53 in the AG adult flies. The control (AG flies at 18 °C) p53 protein level is set as 1, and the indicated genotype flies relative to the control are shown. Trial n = 3. (c) Accumulation of p53 in the larval AG brain. The images show immunostaining with anti-p53 and anti-GluR1 antibodies. The nuclei are stained with DAPI. The result showed that the 53 protein (the red channel) was lower in the control (Appl-Gal4) ventral nerve cord of larval flies; whereas p53 protein level was higher in the AG flies, especially in neurons with higher GluR1 expression (the green channel). Trial n = 2. (d) Effect of p53 on AG fly survival. The survival rate of indicated genotype of flies is shown. Trial n = 4.