Fig. 4.

KI effects on mitochondrial swelling. Rat liver mitochondria were supplemented with 25 µM calcium chloride and incubated with KIs at 100-fold Cmax or the highest testable concentrations as indicated. The six KIs with a black box warning for hepatotoxicity were highlighted in a red box. Mitochondrial swelling was determined by monitoring the decrease in absorbance at 535 nm every 2 min for 30 min. c Shows the dose response of five KIs. a, c Data are means and standard derivations of three separate experiments. *p < 0.05 as compared to DMSO-treated samples at the end of experiments. b Data are means of two independent experiments (color figure online)