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. 2017 Jul 18;7:5739. doi: 10.1038/s41598-017-06016-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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BIL4 is important for cell elongation. (a) qRT-PCR analysis of BIL4 expression in wild-type (WT), BIL4-RNAi-1 (RNAi-1) and BIL4-RNAi-2 (RNAi-2) seedlings grown in the dark for 3 days. The results are presented as the mean ± s.d. (b,c) Three-week-old (b) and 5-week-old (c) wild-type and BIL4-RNAi plants. (d,e) Phenotype (d) and hypocotyl length (e) of wild-type and BIL4-RNAi seedlings grown on medium containing 3 µM Brz in the dark for 6 days. The results are presented as the mean ± s.d., n > 30 seedlings. Asterisks indicate a significant difference from the wild-type plant (P < 0.01 by Student’s t-test). (f to i) SEM images (f,h) and length (g,i) of the hypocotyl cells. The typical cell phenotype is marked by a white frame. Scale bar, 100 µm. The results are presented as the mean ± s.e.m. Asterisks indicate a significant difference relative to wild-type plants (P < 0.01 by Student’s t-test). RNAi seedlings were germinated on 1 µM Brz (n > 17 cells; f,g), and overexpressing seedlings were germinated on 3 µM Brz (n > 35 cells; h and i) in the dark for 7 days.