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. 2017 Jul 18;7:5739. doi: 10.1038/s41598-017-06016-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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BIL4 positively affects the size of leaf epidermal cells. (a) Rosette leaves of wild-type, BIL4-OX and BIL4-RNAi-2 (RNAi-2) plants 6 weeks after sowing. (b) Leaf area of wild-type, BIL4-OX and RNAi-2 plants. The results are presented as the mean ± s.d., n = 5 leaves. Asterisks indicate a significant difference from the wild-type plants (P < 0.01 by Student’s t-test). (c) Confocal images of the epidermis of 6-week-old wild-type, BIL4-OX and RNAi-2 rosette leaves stained with PI. Scale bar, 50 μm. (d) Cell size in the epidermis of wild-type, BIL4-OX and RNAi-2 rosette leaves. The results are presented as the mean ± s.d., n = 9 areas. Asterisks indicate a significant difference from the wild-type plant (P < 0.01 by Student’s t-test). (e) Epidermal cell number of wild-type, BIL4-OX and RNAi-2 rosette leaves. The results are presented as the mean ± s.d., n = 5 leaves; n.s. indicates no significant difference from the wild-type plants (by Student’s t-test).