Fig. 6.

Overexpression of CST1 promotes proliferation in breast cancer cells. a Expression levels of CST1 protein after stable transfection with vector or CST1-expressing plasmids were detected by western blotting analysis. b Cell proliferation after CST1 overexpression in BT-549 cells was measured by MTT assay. c Cell proliferation after CST1 overexpression in MDA-MB-415 cells was measured by MTT assay. d Cell colony formation was determined in BT-549 and MDA-MB-415 cells expressing vector or CST1. e The number of colonies of BT-549 and MDA-MB-415 cells harboring vector or CST1. f The representative pictures of proliferation-related proteins after overexpression of CST1 in BT-549 and MDA-MB-415 cells, as determined by western blotting. Results are expressed as means ± SD (error bars). *p < 0.05, **p < 0.01, and ***p < 0.001 compared to vector