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Construction of recombinant plasmid pUC19-HPK4160::EryBII. The red arrow represents the HPK4160 gene from L. bulgaricus ATCC BAA-365; The cyan arrow represents the ampicillin resistance gene; The purple arrow represents the erythromycin resistance gene; SphI, EcorI, BfaI, BstEII are four endonuclease hydrolysis sites.
