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Recombinant plasmid mapping. 1: PCR detection of the HPK4160::EryBII gene from pUC19-HPK4160::EryBII using Primers 1 and 2; 2: pUC19-HPK4160::EryBII was cut by SphI and EcoRI to 4.4, 2.7, 1.7 kbp fragments; 3: DNA marker III (200, 500, 800, 1,200, 2,000, 3,000, 4,500 bp); 4: pUC19-HPK0115::EryBII was cut by Sph I and EcoR I to 4.7, 2.7, 2.0 kbp fragments. 5: PCR detection of the HPK0115::EryBII gene from pUC19-HPK0115::EryBII using Primers 5 and 6.
