Table 1.
Bacterial strains and plasmids used in this study.
| Strain or plasmid | Relevant genotype or description | Reference and/or source |
|---|---|---|
| Strains E. coli DH5α | F−, φ80d lacZ ΔM15, Δ(lacZYA-argF)U169, deoR, recA1, endA1, hsdR17 (rk−,mk+), phoA, supE44, λ−, thi-1, gyrA96, relA1 | TaKaRa |
| L. bulgaricus ATCC BAA-365 | Wild-type L. bulgaricus | ATCC |
| L. bulgaricus ΔH4160-1 | LBUL_RS04160 gene mutant of L. bulgaricus ATCC BAA-365; HPK4160::EryB | This study |
| L. bulgaricus ΔH0115-1 | LBUL_RS00115 gene mutant of L. bulgaricus ATCC BAA-365; HPK0115::EryB | This study |
| L. bulgaricus rH0115-1 | Complementation of the LBUL_RS00115 mutant with pMG56e carrying LBUL_RS00115 gene | This study |
| S. cerevisiae Y2HGOLD | the HIS3, ADE2, and MEL1/AUR1-C reporter genes are under the control of Gal4-responsive promoter elements-G1, G2, and M1 | Clontech |
| Plasmids | ||
| pMD18T | clone vector; Ampr | TaKaRa |
| pUC19 | clone vector; Ampr | TaKaRa |
| pMG76e | Expression vector of lactic acid bacteria; Emr | College of food science and Nutritional Engineering, China Agricultural University |
| pMG56e | Expression vector of lactic acid bacteria; Cmr, derivative of pMG36e in which the gene coding for erythromycin resistance was replaced with a gene coding for chloramphenicol resistance from pNZ8148 | College of food science and Nutritional Engineering, China Agricultural University |
| pUC19-HPK4160 | pUC19 derived integration vector containing the LBUL RS04160 gene with SphI, EcoRI restriction enzyme sites; Ampr | This study |
| pUC19-HPK4160::EryBII | pUC19-HPK4160 derived integration vector containing the EryBII gene; Emr; Ampr | This study |
| pUC19-HPK0115 | pUC19 derived integration vector containing the LBUL RS00115 gene with SphI, EcoRI restriction enzyme sites; Ampr | This study |
| pUC19-HPK0115::EryBII | pUC19-HPK0115 derived integration vector containing the EryBII gene; Emr; Ampr | This study |
| pMG56e-HPK0115 | pMG56e derived expression vector carrying full LBUL_RS00115 gene; Cmr | This study |
| pGBKT7 | Plasmid in yeast two-hybrid system; Gal4 (1–147), Trp1, Kanr | Clontech |
| pGADT7 | Plasmid in yeast two-hybrid system; Gal4 (768–881), Leu2, Ampr | Clontech |
| pGBKT7-53 | Positive control plasmid that encodes a fusion of the murine p53protein (72–390) and the GAL4 DNA-BD (1–147) | Clontech |
| pGBKT7-Lam | Negative control plasmid that encodes the Gal4 BD fused with lamin | Clontech |
| pGADT7-T | Positive control plasmid that encodes a fusion of the SV40 large T antigen (87–708) and the GAL4 AD (768–881) | Clontech |
| pGBK-HPK0115 | pGBKT7 derived expression vector carrying full LBUL_RS00115 gene; Kanr | This study |
| pGAD-RR0110 | pGADT7 derived expression vector carrying full LBUL_RS00110 gene; Ampr | This study |