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. 2017 Jul 18;7:5754. doi: 10.1038/s41598-017-06209-9

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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p32 ^+/− mice demonstrate increased caloric intake and energy expenditure. (A) Caloric intake in 52-week-old mice fed NC (n = 5). Caloric intake is normalized to body weight. (B) Fecal lipid excretion in 52-week-old mice fed NC (n = 5). (C) Heat release in 52-week-old mice fed NC (n = 3). (D) Caloric intake in mice fed an HFD (WT, n = 7; p32 ^+/−, n = 5). Caloric intake is normalized to body weight. (E) Heat release in WT and p32 ^+/− mice fed an HFD for ten weeks (n = 3). Statistical comparisons were evaluated by two-way-ANOVA for the indirect calorimetry assay.