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. 2017 Jul 18;7:5756. doi: 10.1038/s41598-017-05810-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Non-heme iron levels in serum, liver and heart of Hfe ^+/+ and Hfe ^−/− mice. Non-heme iron content in serum (A), liver (B) and heart (C) was measured colorimetrically using bathophenanthroline (n = 5–9 per group). Data are presented as the means ± SEM. *p < 0.05 between Hfe ^−/− and Hfe ^+/+ mice assessed by the two-way ANOVA, followed by the Tukey’s post-hoc analysis.