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. 2017 Jul 18;7:5759. doi: 10.1038/s41598-017-05977-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Tumorigenic effect of E6-induced Axl expression in cervical cancer cells. (a) Representative images of transwell invasion assay after transfection of HE6F cells with scramble and Axl siRNA (upper panel). Scale bars, 100 μm. The percentages of invaded cells were calculated from the images in bar graph (lower panel). (b) HPV16E6-mediated Axl expression modulates susceptibility to NK cell cytotoxicity in HE6F cells. Cytolysis activity was determined by an LDH release assay at the indicated effector:target (E:T) ratios. Data in b are shown as the mean ± s.e.m. (***P < 0.001). Data are representative of five independent experiments. (c) HE6F cells treated with sFasL and transfected with Axl siRNA were analysed for apoptosis by flow cytometry using annexin V-FITC and propidium iodide (PI) staining. The numbers in each quadrant represent the percentage of cells positive for annexin V and/or PI. (d) Expression of apoptosis-related genes was analysed by RT-PCR in Axl-silenced HE6F cells compared to scrambled siRNA-transfected cells.