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. 2017 Jul 18;7:5654. doi: 10.1038/s41598-017-05970-1

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of radiation in cell turnover, OB volume and mitral cells. (A,B) Coronal slices of the OB from control and irradiated mice 15 days after BrdU injection; note the reduction in BrdU staining; inset panels enlarge representative areas to show the differences between both situations. (C,D) Estimation of BrdU-positive cells in the different OB layers 15 (C) and 60 days (D) after BrdU injection; note the reduction in the number of cells in the irradiated animals except for the EPL 15 days after BrdU injection. (E) Estimation of the OB volume; note the dramatic reduction in irradiated animals at both survival times. (F,G) Mitral cells of both experimental groups stained with reelin (green); no differences were detected. M, medial; D, dorsal. *p < 0.05; **p < 0.01. Scale bar 500 μm for (A,B); 100 μm for (F,G).