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. 2017 Jul 18;7:5654. doi: 10.1038/s41598-017-05970-1

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Cell characterization. (A,B) Focal planes with 3D stacks showing co-localization between BrdU (green) and tyrosine hydroxylase (TH, red; A) or somatostatin (SOM, red; B); inset panels show a higher magnification of double-labelled cells; images correspond to control mice of 60 days of survival. (C,D) Charts showing the percentages of co-localization of BrdU and the former markers; cells positives for both BrdU and TH appeared only in the GL 60 days after BrdU injection (C) BrdU-SOM positive cells were detected in the EPL (D) no differences were found between experimental groups for both markers at any survival time or bulbar layer. Scale bar 20 μm.