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. 2017 Jul 19;11:218. doi: 10.3389/fncel.2017.00218

Figure 4.

Figure 4

Involvement of JNK1/2 in MMP-9 induction in primary cultured astrocytes exposed to 2-chloroethanol. Notes: SP, SP600125; 2-CE, 2-chloroethanol. Cells in the exposure group and intervention groups were exposed to 30 mM 2-CE for 24 h. Cells in the intervention groups were pre-exposed with SP 1 h before 2-CE exposure. (A) Western blot analysis. Images were the representative results of four separate experiments for each group. (B) Densitometric analysis of western blots. The relative intensity of MMP-9 and p-JNK1/2 in arbitrary units was compared to β-actin. The ratios of phosphorylated JNK1/2 to native JNK1/2 was expressed as the p-JNK1/JNK1 and p-JNK2/JNK2. (C) Quantitation of mRNA by real-time RT-PCR. The gene expression was normalized to GAPDH and presented as fold change vs. the control. Data were expressed as mean ± SD of four independent experiments performed on four batches of primary cultured astrocytes, and analyzed by One-way ANOVA. Significant difference was defined as p < 0.05, and *, vs. blank control; #, vs. 30 mM 2-CE exposure group; +, vs. 0.1 μM SP inhibition group; &, vs. 1 μM SP inhibition group.