Table 2.
Total lipid content (% of biomass) and EPA distribution (% of total fatty acids) in control (22°C) and stressed cultures (10°C) of P. tricornutum after 1, 2, 4 and 8 days of temperature stress. For the methods used for lipid extraction and analysis, see the electronic supplementary material, SD1. After a one-way ANOVA used in analysis of the influence of temperature stress, SNK multiple comparison test results are arranged in increasing order from left to right: a < b < c (p < 0.05).
| EPA level |
||||
|---|---|---|---|---|
| day | stress temperature (°C) | lipid content (% biomass) | total lipids | galactolipids |
| 1 | 22 | 7.8±1.3a | 32.1±1.6a | 40.2±1.8ab |
| 10 | 14.3±1.8c | 31.6±2.3a | 42.4±2.2b | |
| 2 | 22 | 8.2±0.6a | 32.9±1.4a | 39.4±1.1ab |
| 10 | 7.4±0.9a | 38.7±2.1b | 50.9±2.5c | |
| 4 | 22 | 8.0±2.1a | 29.6±2.9a | 39.1±1.8ab |
| 10 | 9.3±1.1ab | 40.7±0.2b | 50.0±0.21c | |
| 8 | 22 | 10.7±0.7b | 33.1±1.6a | 37.1±4.3a |
| 10 | 9.4±1.3ab | 40.6±3.0b | 50.1±6.2c | |