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. 2017 Jul 19;7:326. doi: 10.3389/fcimb.2017.00326

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Copyright © 2017 Grzymajlo, Ugorski, Suchanski, Kedzierska, Kolenda, Jarzab, Biernatowska and Schierack.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Adherence and invasion of S. Choleraesuis isogenic mutant strains to enterocytes with overexpression (IPEC-CRT cells), suppressed expression (IPEC-shCRT cells) or neoexpression (ICE-CRT) of calreticulin. (A) Adhesion assay with IPEC-PURO cells and IPEC-CRT cells. (B) Adhesion assay with IPEC-shPURO cells and IPEC-shCRT cells. (C) Invasion assay with IPEC-PURO and IPEC-CRT cells. (D) Invasion assay with IPEC-shPURO and IPEC-shCRT cells. (E) Adhesion assay with ICE-PURO cells and ICE-CRT cells. (F) Invasion assay with ICE-PURO and ICE-CRT cells. Data are means ± SD in triplicate assays, which were independently repeated three times.