Table 2.
List of primers and probes for EHDV pan‐reactive and type‐specific assays
| Assay type | Oligo name | Oligo sequence (5′–3′) |
|---|---|---|
| Seg‐9‐based/group‐specific | ||
| EHDV/Seg‐9/F/15‐32 | ATGTCAGCTGCGGTYTTG | |
| EHDV/Seg‐9/R/112‐85 | TCCCAATCAACTAARTGRATYTGVATCT | |
| EHDV/Seg‐9/P/69‐48 | CCTCGGTCGAACGTTGGATCAC | |
| Seg‐2‐based/type‐specific | ||
| EHDV‐1w/Seg‐2/246‐275F | GAATAATTCGYTAYGAGAATAAARCYAAAG | |
| EHDV‐1w/Seg‐2/364‐341R | TCTATGYGYCTCRTCCATTCTYGG | |
| EHDV‐1wSeg‐2/305‐329P | CAGCTGCGGTCATCTATTAGGCATC | |
| EHDV‐1e/Seg‐2/260‐278F | GACAGCAAAAGTAGAGGAG | |
| EHDV‐1e/Seg‐2/359‐341R | GGGTTTCATCCATTTTTGG | |
| EHDV‐1e/Seg‐2/304‐329P | CGAGTTACGATCATCTATCAGGCATC | |
| EHDV‐2e/Seg‐2/2540‐2559F | GAAGTATTGGTTAAATATCG | |
| EHDV‐2e/Seg‐2/2627‐2606R | GCTRAAATGCGTATTCAATGG | |
| EHDV‐2e/Seg‐2/2564‐2590P | CCCACGCAGGGAGATCACCGAYTYAAC | |
| EHDV‐2w/Seg‐2/1910‐1934F | TATGTTAAATGTATTGAATTATACG | |
| EHDV‐2w/Seg‐2/2087‐2069R | TCTCATCCCGACCAACACT | |
| EHDV‐2w/Seg‐2/2027‐1997P | CTCTTCATCCGGATCCTGATATACCTCCATC | |
| EHDV‐4w/Seg‐2/339‐360F | GGACTTTGAATCATTGATGTTG | |
| EHDV‐4wSeg‐2/445‐426R | GCACGTCAGTTTGCTGCAGT | |
| EHDV‐4w/Seg‐2/415‐387P | CTCGCCGCCCTGTGAAGTCAACTCCTGCC | |
| EHDV‐5w/Seg‐2/783‐798F | TGGTGAGCGTGGTGCG | |
| EHDV‐5w/Seg‐2/863‐839R | GCAGCTATATCATCTAAAGCAATTG | |
| EHDV‐5w/Seg‐2/809‐830P | CACGCGAAGGAATAGCCCCATC | |
| EHDV‐6e/Seg‐2/601‐622F | GATTGTAATAGGAGAGATTAAG | |
| EHDV‐6e/Seg‐2/743‐724R | GACCCAAAGCCGCCTGGATT | |
| EHDV‐6e/Seg‐2/638‐669P | CGTCAAAATGTCATAACTCGGCAGATGATACC | |
| EHDV‐7e/Seg‐2/212‐229F | GATGGGCTATGATATCAT | |
| EHDV‐7e/Seg‐2/323‐304R | TAATCCCTGTTCTTCACCTT | |
| EHDV‐7e/Seg‐2/280‐301P | CCAAGAACTCAAAGGATCCGGC | |
| EHDV‐7w/Seg‐2/2134‐2150F | GACAAATTTTGATGCCG | |
| EHDV‐7w/Seg‐2/2241‐2225R | GAGAAAAGTTGGGCGCT | |
| EHDV‐7w/Seg‐2/2220‐2194P | CCTCTAAGATCTCATCCCGTCTCTCCC | |
| EHDV‐8e/Seg‐2/886‐900F | GCGGATCGAAGAAAT | |
| EHDV‐8e/Seg‐2/1021‐1001R | AGTGGTCTTAACCAAGTTCTG | |
| EHDV‐8e/Seg‐2/992‐966P | CGTTATACAATCTGACTCGCGCCCCCC | |
| EHDV‐9w/S2/2147‐2169F | ACTAAATGAAGAAGAGATACGTA | |
| EHDV‐9w/S2/2251‐2228R | GCTATAATGTTATAGAAATTTGGT | |
| EHDV‐9w/S2/2192‐2226P | CTAATGCCCGCATTATTGCTTCCCGATGGT | |
The names of the oligonucleotide primers and probes indicate the group‐ or type‐specificity of the assay. Seg‐9 indicates the group‐specific assays while Seg‐2 indicates respective type‐specific assays. F, R and P stand for ‘forward primer’, ‘reverse primer’ and ‘probe’, respectively. The numbers represent annealing positions on the genome segment.