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RT‐PCR assay composition
| Reagent | Assay detecting segment | |
|---|---|---|
| Seg‐9 | Seg‐2 | |
| Forward primer (μl) | 1 (15 μ m) | 1 (10 μ m) |
| Reverse primer (μl) | 1 (15 μ m) | 1 (10 μ m) |
| Probe (μl) | 0.5 (5 μ m) | 0.5 (5 μ m) |
| 50 mM MgSO4a (μl) | 1 | 1 |
| SuperScript III RT/Platinum Taq Mixa (μl) | 0.5 | 0.5 |
| 2× Reaction Mixa (μl) | 12.5 | 12.5 |
| Nuclease free water (μl) | 4.5 | 4.5 |
| dsRNA(μl) | 4.0 | 4 |
| Total volume(μl) | 25 | 25 |
SuperScript III/Platinum Taq one‐step qRT‐PCR kit (Invitrogen).
