Table 5.
Specificity of EHDV group‐specific (Seg‐9) and type specific (Seg‐2) assays
| Virus species‐serotype | Origin | ORC reference number | Ct values | |
|---|---|---|---|---|
| Seg‐9 | Seg‐2 | |||
| EHDV‐1w | USA | USA1955/01 | 15.60 | 18.39 |
| EHDV‐1w | Nigeria | NIG1967/01 | 16.79 | 19.32 |
| EHDV‐2w | Canada | CAN1962/01 | 19.01 | 16.37 |
| EHDV‐2e | Japan | JAP1959/01 | 19.34 | 17.66 |
| EHDV‐2e | Australia | AUS1979/05 | 21.10 | 17.72 |
| EHDV‐4w | Nigeria | NIG1968/01 | 19.40 | 19.10 |
| EHDV‐5e | Australia | AUS1977/01 | 19.60 | 18.50 |
| EHDV‐6e | Australia | AUS1981/07 | 16.50 | 21.70 |
| EHDV‐6w | Bahrain | BAR1983/01 | 18.23 | 22.41 |
| EHDV‐7e | Australia | AUS1981/06 | 18.60 | 20.15 |
| EHDV‐7w | Israel | ISR2006/013 | 16.49 | 21.03 |
| EHDV‐8e | Australia | AUS1982/06 | 15.70 | 13.14 |
| EHDV‐9w | RSA | – | – | – |
| AHSV‐1 | RSA | RSArah1/03 | No Ct | No Ct |
| AHSV‐2 | RSA | RSArah2/03 | No Ct | No Ct |
| AHSV‐3 | RSA | RSArah3/03 | No Ct | No Ct |
| AHSV‐4 | RSA | SPArah4/03 | No Ct | No Ct |
| AHSV‐5 | RSA | RSArah5/03 | No Ct | No Ct |
| AHSV‐6 | RSA | RSArah6/03 | No Ct | No Ct |
| AHSV‐7 | Kenya | KENrah7/03 | No Ct | No Ct |
| AHSV‐8 | RSA | RSArah8/03 | No Ct | No Ct |
| AHSV‐9 | Pakistan | PAKrah9/03 | No Ct | No Ct |
| EEV‐1 | RSA | RSA1967/03 | No Ct | No Ct |
| EEV‐2 | RSA | RSA1971/06 | No Ct | No Ct |
| EEV‐3 | RSA | RSA1974/06 | No Ct | No Ct |
| EEV‐4 | RSA | RSA1976/03 | No Ct | No Ct |
| EEV‐5 | RSA | RSA1976/06 | No Ct | No Ct |
| EEV‐6 | RSA | RSA1991/03 | No Ct | No Ct |
| PHSV | Peru | PER1997/01 | No Ct | No Ct |
| BTV‐1 | RSA | RSArrrr/01 | No Ct | No Ct |
| BTV‐2 | RSA | RSArrrr/02 | No Ct | No Ct |
| BTV‐3 | RSA | RSArrrr/03 | No Ct | No Ct |
| BTV‐4 | RSA | RSArrrr/04 | No Ct | No Ct |
| BTV‐5 | RSA | RSArrrr/05 | No Ct | No Ct |
| BTV‐6 | RSA | RSArrrr/06 | No Ct | No Ct |
| BTV‐7 | RSA | RSArrrr/07 | No Ct | No Ct |
| BTV‐8 | RSA | RSArrrr/08 | No Ct | No Ct |
| BTV‐9 | RSA | RSArrrr/09 | No Ct | No Ct |
| BTV‐10 | RSA | RSArrrr/10 | No Ct | No Ct |
| BTV‐11 | RSA | RSArrrr/11 | No Ct | No Ct |
| BTV‐12 | RSA | RSArrrr/12 | No Ct | No Ct |
| BTV‐13 | RSA | RSArrrr/13 | No Ct | No Ct |
| BTV‐14 | RSA | RSArrrr/14 | No Ct | No Ct |
| BTV‐15 | RSA | RSArrrr/15 | No Ct | No Ct |
| BTV‐16 | RSA (originally from Pakistan) | RSArrrr/16 | No Ct | No Ct |
| BTV‐17 | RSA | RSArrrr/17 | No Ct | No Ct |
| BTV‐18 | RSA | RSArrrr/18 | No Ct | No Ct |
| BTV‐19 | RSA | RSArrrr/19 | No Ct | No Ct |
| BTV‐20 | RSA | RSArrrr/20 | No Ct | No Ct |
| BTV‐21 | RSA | RSArrrr/21 | No Ct | No Ct |
| BTV‐22 | RSA | RSArrrr/22 | No Ct | No Ct |
| BTV‐23 | RSA | RSArrrr/23 | No Ct | No Ct |
| BTV‐24 | RSA | RSArrrr/24 | No Ct | No Ct |
| BTV‐25 | Switzerland | SWI2008/01 | No Ct | No Ct |
| BTV‐26 | Kuwait | KUW2010/02 | No Ct | No Ct |
| BTV‐27 | Corsica, France | strain 37 | No Ct | No Ct |
| BTV‐28 | Middle‐east | – | No Ct | No Ct |
| BTV‐29 | RSA | – | No Ct | No Ct |
| C. sonorensis | No Ct | No Ct | ||
| Uninfected BHK cells | No Ct | No Ct | ||
| Uninfected Vero cells | No Ct | No Ct | ||
| Uninfected KC cells | No Ct | No Ct | ||
| Uninfected cattle blood | No Ct | No Ct | ||
Representatives of different serotypes and topotypes from five different Orbivirus species (AHSV, EEV, PHSV, EHDV and BTV) were tested to confirm the specificity of assays for EHDV dsRNA. Further details on these isolates can be obtained from ORC http://www.reoviridae.org/dsRNA_virus_proteins/ReoID/viruses-at-iah.htm. EEV = Equine encephalosis virus; PHSV = Peruvian horse sickness virus; EHDV = Epizootic haemorrhagic disease virus; BTV = Bluetongue virus. RSA = Republic of South Africa.