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. 2017 Mar 13;19(8):e12735. doi: 10.1111/cmi.12735

Figure 1.

Figure 1

Laser capture microdissection (LCM) efficiently isolates liver stages of P. cynomolgi enabling high throughput transcriptomic analyses of these stages. (a) Top: Cresyl violet staining of a Plasmodium cynomolgi‐infected monkey hepatocyte culture with a schizont form indicated (left). Bottom: Immunofluorescence microscopy of a P. cynomolgi‐infected monkey hepatocyte culture stained with anti‐parasite HSP70 antibodies identifies hypnozoite forms (left). The area selected for LCM is indicated before (middle) and after (right) dissection. (b) The percentage of reads that mapped to either the P. cynomolgi or the M. fascicularis genomes are indicated for each sample. (d) position of the samples in the space spanned by the first two components generated from a principal component analysis of the log2(read counts) data. For parts B‐C, hypnozoite = H1 and H2; liver schizont = S1 and S2; blood stage = BS1 and BS2