Figure 3.

CMs from hDPCs enhance endothelial cell tube formation in vitro. The angiogenic potentials of CMs were tested by Matrigel tube formation assay (n = 6); HUVECs were suspended in CM–Nor or CM–Hyp in duplicate; tube formation was also observed after culture in serum‐free DMEM and EGM‐2 Bullet Kit as negative and positive controls (data not shown), respectively; photographs are from (A) control, (B) CM–Nor and (C) CM–Hyp cultures. Number of branch points (D), total tube length (E) and tube formation area (F) were calculated using NIS elements software: the CM–Nor cultures showed higher tube formation than the control cultures; the CM–Hyp cultures showed greater angiogenic potential than CM–Nor cultures; data represent mean ± SD; **p < 0.01, *p < 0.05; bar = 200 µm. [Colour figure can be viewed at wileyonlinelibrary.com]