Figure 4.

CM from hypoxic conditions promotes endothelial cell migration in vitro. Wound‐healing assays were performed to assess the angiogenic potentials of CMs from hDPCs. HUVECs were cultured in Culture‐Insert 24‐well plates, in duplicate; after the cells had reached 80% confluence, the Culture‐Insert was gently removed; the wounded monolayers were incubated with CM–Nor (B) and CM–Hyp (C) and both positive (data not shown) and negative (A) control media. After 24 h of incubation, the cells were fixed and stained with crystal violet; the percentage of wound closure was calculated using ImageJ software. (D) CM–Hyp cultures showed greater angiogenic potential compared with the others; data represent mean ± SD; **p < 0.01; bar = 250 µm; line of black dots represents base line