Figure 2.

Measuring the effect of rapamycin on apoptosis in MDSPCs isolated from progeroid Ercc1 ^−/Δ mice. (A) Representative images of TUNEL assay. Live cells (DAPI: blue), apoptotic cells (yellow–green), and merged figures. Scale bar = 100 µm. (B) The percentage of TUNEL‐positive cells was counted from images obtained from four independent MDSPC populations per genotype and treatment group. Error bars indicate the standard deviation. ^### p < 0.001 versus WT‐DMSO, ***p < 0.001 versus ERCC1 ^−/Δ‐DMSO. Statistical significance was determined using the Kruskal–Wallis test with Scheffe's post hoc test. (C) Immunoblotting to measure the expression of cleaved PARP, an apoptosis‐related marker, in MDSPCs isolated from Ercc1 ^−/Δ and WT mice cultured with and without rapamycin.