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Workflow for the Whole Blood Nuclear Localization Kit. A) The workflow for the Nuclear Localization Kit is similar to most Fix/Perm kits for flow cytometry, comprised of a brief fixation step, followed by permeabilization and then labeling. B) What differentiates the Nuclear Localization Kit is that 2 aliquots of the sample are permeabilized side‐by‐side with 2 different lysis buffers, enabling the nuclear signal to be quantitatively calculated from the resulting data.
