Simultaneous loss of ERG and Friend leukemia integration 1 transcription factor (FLI1) in human pulmonary artery endothelial cells (HPAECs) synergistically induces the expression of inflammatory and IFN genes. HPAECs were subjected to small interfering RNA (siRNA)-mediated silencing of FLI1 and/or ERG for 48 hours. siFE indicates double FLI1 and ERG siRNA treatment. Scrambled siRNA (siSCR) was used as a control. Relative mRNA expression was determined by quantitative RT-PCR (n = 3 independent experiments). Data shown as mean (±SD). One-way ANOVA followed by Tukey’s multiple comparison were used for statistical analysis. *P < 0.05, **P < 0.01, ***P < 0.001. CXCL 10, chemokine (C-X-C motif) ligand 10; CX3CL1, chemokine (C-X3-C motif) ligand 1; GBP1, guanylate-binding protein 1; HAS2, hyaluronan synthase 2; IFI44L, IFN-induced protein 44-like; IFNB1, interferon β 1; IRF, IFN-regulatory factors.