Figure 5.
ACTA2-CreERT2 lineage-traced myoepithelial progenitors give rise to other submucosal gland cell types. ACTA2-CreERT2:ROSA-TG mice were induced on the day of birth, and 21-day-old tracheal samples were then immunofluorescently stained with each of the indicated cellular markers, GFP, and tdTomato. DAPI was used to mark nuclei. (A) Lysozyme staining was used to identify serous cells. (B) Dolichos biflorus agglutinin (DBA) lectin staining was also used to identify serous cells. (C) Ulex europaeus agglutinin I (UEA-1) lectin staining was used to identify mucin within glandular mucous tubules. (D) Tumor-associated calcium signal transducer 2 (Trop-2) staining was used to identify duct cells. The boxed region in the main left panel is enlarged to the right as multi- and single-channel images. Scale bars: 25 μm.