Figure 3.
Selective knockdowns of certain Gα subunits in HASM attenuate TAS2R signaling to [Ca2+]i. (A) Representative [Ca2+]i tracings in response to 250 μM DPD after transfection of immortalized HASM cells with control (scrambled) or α subunit–specific small interfering RNA (siRNA), with the inset showing a representative Western blot indicating the degree of α subunit knockdown. (B) Mean (±SE) of the peak [Ca2+]i response to 250 μM DPD from four experiments. *P < 0.01 versus control siRNA. (C) Peak [Ca2+]i DPD responses to various combinations of Gαi1, Gαi2, or Gαi3 knockdowns. *P < 0.01 versus control siRNA, †P < 0.05 versus all other combinations (n = 5–6).