Table 2.
Cross-amplification of Brassica-derived SSR markers across seven species of Brassicaceae family
| Sr.no. | Marker ID | Number of alleles | PIC value | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| B. nigra | B. juncea | B. rapa | B. napus | B. carinata | Eruca sativa | B. oleracea L. | ||||||
| ssp. toria | ssp. Yellow Sarson | ssp. Brown sarson | var. botrytis | var. capitata | ||||||||
| A) B. nigra-derived SSRs | ||||||||||||
| 1 | SB1937 | 1 | 1 | 2 | 3 | 2 | 3 | 1 | 1 | 3 | 3 | 0.54 |
| 2 | SJ3627R | 1 | 1 | 2 | 2 | 2 | 2 | 2 | 1 | 2 | 4 | 0.72 |
| 3 | SA0306 | 2 | 3 | 3 | 3 | 3 | 3 | 3 | 2 | 2 | 3 | 0.62 |
| 4 | SJ84165 | 1 | 1 | 1 | 2 | 2 | 3 | 1 | 3 | 2 | 3 | 0.64 |
| 5 | SJ03104 | 2 | 1 | 1 | 1 | 1 | 1 | 2 | 2 | 1 | 1 | 0.57 |
| 6 | SJ6842 | 2 | 1 | 2 | 3 | 2 | 4 | 2 | 3 | 2 | 2 | 0.75 |
| 7 | SJ1536 | 1 | 1 | 1 | 1 | 1 | 1 | 2 | 1 | 1 | 1 | 0.04 |
| 8 | SB0372 | 1 | 1 | 2 | 2 | 2 | 2 | 1 | 2 | 2 | 2 | 0.47 |
| 9 | SB31138 | 1 | 1 | 3 | 1 | 1 | 2 | 2 | 2 | 2 | 2 | 0.45 |
| 10 | SJ3874I | 2 | 2 | 2 | 1 | 2 | 1 | 1 | 1 | 1 | 1 | 0.37 |
| 11 | SJ34121 | 2 | 1 | 1 | 2 | 1 | 2 | 3 | 2 | 2 | 2 | 0.46 |
| 12 | SB3140 | 2 | 2 | 2 | 2 | 2 | 2 | 1 | 2 | 2 | 2 | 0.56 |
| 13 | SJ3640I | 1 | 1 | 1 | 1 | 2 | 2 | 2 | 2 | 1 | 1 | 0.23 |
| 14 | SJ6846 | 2 | 1 | 2 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0.7 |
| 15 | SJ8033 | 4 | 2 | 2 | 3 | 2 | 3 | 3 | 2 | 3 | 3 | 0.76 |
| 16 | SJ0338 | 2 | 1 | 2 | 2 | 3 | 2 | 2 | 3 | 2 | 3 | 0.67 |
| 17 | SB2141AI | 1 | 1 | 1 | 1 | 1 | 1 | 2 | 1 | 1 | 1 | 0.16 |
| 18 | SJ7079 | 2 | 1 | 1 | 1 | 1 | 1 | 1 | 2 | 1 | 0 | 0.39 |
| 19 | SJ39119I | 2 | 1 | 3 | 3 | 3 | 3 | 1 | 2 | 4 | 3 | 0.65 |
| 20 | SB0202I | 3 | 3 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 0.61 |
| 21 | SJ3627R | 1 | 1 | 1 | 1 | 1 | 2 | 2 | 1 | 2 | 4 | 0.71 |
| 22 | SB2131 | 2 | 1 | 1 | 1 | 2 | 1 | 1 | 2 | 3 | 2 | 0.64 |
| 23 | SJ4933 | 1 | 1 | 1 | 1 | 2 | 1 | 1 | 1 | 2 | 1 | 0.56 |
| 24 | SB05631 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | - |
| 25 | SJ3838 | 2 | 2 | 1 | 2 | 2 | 2 | 2 | 1 | 1 | 1 | 0.47 |
| 26 | SJ3302RI | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | - |
| 27 | Ni4F09 | 2 | 2 | 1 | 1 | 2 | 2 | 2 | 2 | 1 | 1 | 0.50 |
| 28 | Ni2A12 | 2 | 1 | 1 | 1 | 1 | 1 | 1 | 2 | 1 | 1 | 0.53 |
| 29 | Ni2A02 | 2 | 1 | 1 | 1 | 1 | 1 | 3 | 1 | 1 | 1 | 0.40 |
| 30 | Ni3C05 | 1 | 3 | 2 | 3 | 4 | 1 | 3 | 4 | 2 | 2 | 0.74 |
| 31 | Ni2D10 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | - |
| B) B. rapa-derived SSRs | ||||||||||||
| 32 | BrgMS418 | 2 | 2 | 2 | 1 | 1 | 3 | 3 | 2 | 3 | 3 | 0.72 |
| 33 | BrgMS68 | 2 | 1 | 1 | 1 | 2 | 1 | 1 | 2 | 1 | 2 | 0.32 |
| 34 | BrgMS75 | 1 | 1 | 2 | 2 | 2 | 2 | 4 | 3 | 4 | 3 | 0.64 |
| 35 | BrgMS804 | 1 | 1 | 1 | 1 | 1 | 3 | 2 | 1 | 1 | 1 | 0.34 |
| 36 | BrgMS422 | 1 | 1 | 3 | 2 | 2 | 1 | 2 | 1 | 2 | 1 | 0.47 |
| 37 | BrgMS426 | 1 | 1 | 2 | 1 | 3 | 1 | 1 | 1 | 1 | 1 | 0.14 |
| 38 | BrgMS135 | 2 | 1 | 2 | 2 | 2 | 3 | 2 | 2 | 2 | 2 | 0.46 |
| 39 | BrgMS1237 | 1 | 1 | 2 | 1 | 2 | 2 | 1 | 1 | 1 | 1 | 0.62 |
| 40 | BrgMS801 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 2 | 1 | 2 | 0.45 |
| 41 | BrgMS397 | 1 | 1 | 3 | 1 | 2 | 2 | 2 | 0 | 3 | 1 | 0.49 |
| 42 | BrgMS521 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0.25 |
| 43 | BrgMS745 | 1 | 1 | 1 | 1 | 1 | 1 | 2 | 2 | 2 | 3 | 0.35 |
| 44 | BrgMS432 | 1 | 1 | 2 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0.04 |
| 45 | BrgMS792 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 0.5 |
| 46 | BrgMS334 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 0.67 |
| 47 | BrgMS316 | 5 | 3 | 1 | 1 | 1 | 2 | 2 | 4 | 1 | 1 | 0.72 |
| 48 | BrGMS4497 | 3 | 3 | 2 | 2 | 2 | 5 | 6 | 1 | 4 | 5 | 0.76 |
| 49 | BrgMS372 | 2 | 2 | 2 | 1 | 3 | 3 | 1 | 3 | 1 | 1 | 0.65 |
| 50 | BrgMS2766 | 1 | 1 | 1 | 1 | 2 | 1 | 2 | 1 | 1 | 2 | 0.61 |
| 51 | BrgMS751 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | - |
| 52 | BrgMS413 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | - |
| 53 | BrgMS313 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | - |
| 54 | BrgMS359 | 2 | 1 | 1 | 2 | 1 | 2 | 1 | 1 | 2 | 2 | 0.39 |
| 55 | BrgMS793 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | - |
| 56 | BrgMS430 | 1 | 1 | 2 | 1 | 2 | 1 | 1 | 1 | 1 | 1 | 0.33 |
| 57 | BrgMS710 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | - |
| 58 | BrgMS1238 | 1 | 2 | 2 | 1 | 2 | 2 | 2 | 1 | 1 | 1 | 0.46 |
| 59 | BrgMS412 | 1 | 1 | 2 | 1 | 3 | 2 | 1 | 1 | 2 | 1 | 0.41 |
| 60 | BrgMS722 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | - |
| 61 | BrgMS787 | 2 | 2 | 2 | 3 | 3 | 2 | 2 | 1 | 1 | 2 | 0.66 |
| 62 | BrgMS399 | 1 | 1 | 1 | 1 | 1 | 2 | 1 | 1 | 2 | 1 | 0.23 |
| 63 | BrgMS713 | 2 | 2 | 3 | 2 | 4 | 4 | 2 | 1 | 2 | 1 | 0.71 |
| 64 | BrgMS4508 | 2 | 1 | 4 | 2 | 2 | 3 | 2 | 1 | 3 | 2 | 0.72 |
| 65 | BrgMS4533 | 3 | 1 | 2 | 1 | 2 | 2 | 2 | 2 | 1 | 2 | 0.58 |
| 66 | BrgMS4536 | 2 | 2 | 2 | 2 | 2 | 2 | 1 | 1 | 1 | 1 | 0.46 |
| 67 | BrgMS63 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0.09 |
| 68 | BrgMS490 | 1 | 1 | 1 | 1 | 2 | 2 | 1 | 4 | 1 | 3 | 0.59 |
| 69 | BrgMS776 | 1 | 1 | 1 | 1 | 2 | 3 | 3 | 1 | 1 | 2 | 0.37 |
| 70 | BrgMS961 | 1 | 1 | 1 | 1 | 3 | 2 | 1 | 1 | 2 | 2 | 0.52 |
| 71 | BrgMS794 | 2 | 2 | 1 | 1 | 1 | 2 | 1 | 1 | 2 | 3 | 0.69 |
| 72 | BrgMS724 | 1 | 1 | 2 | 2 | 2 | 3 | 1 | 1 | 1 | 1 | 0.31 |
| 73 | BrgMS337 | 2 | 3 | 1 | 1 | 2 | 1 | 1 | 0 | 0 | 1 | 0.57 |
| 74 | BrgMS70 | 4 | 3 | 2 | 1 | 2 | 2 | 0 | 0 | 0 | 0 | 0.71 |
| 75 | BrgMS2767 | 2 | 2 | 2 | 2 | 2 | 1 | 1 | 2 | 2 | 2 | 0.42 |
| 76 | BrgMS778 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0.18 |
| 77 | BrgMS802 | 2 | 1 | 2 | 2 | 1 | 2 | 1 | 1 | 1 | 2 | 0.62 |
| 78 | BrgMS344 | 1 | 1 | 1 | 1 | 2 | 1 | 1 | 1 | 2 | 2 | 0.64 |
| 79 | BrgMS783 | 1 | 1 | 1 | 1 | 2 | 3 | 1 | 1 | 1 | 1 | 0.52 |
| 80 | BrgMS780 | 2 | 1 | 1 | 1 | 1 | 1 | 2 | 1 | 1 | 1 | 0.25 |
| 81 | BrgMS350 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | - |
| 82 | BrgMS388 | 2 | 1 | 2 | 1 | 2 | 4 | 2 | 1 | 4 | 3 | 0.69 |
| 83 | BrgMS60 | 1 | 1 | 1 | 1 | 1 | 2 | 2 | 1 | 1 | 1 | 0.14 |
| 84 | BrgMS516 | 1 | 1 | 1 | 1 | 2 | 2 | 1 | 1 | 1 | 1 | 0.16 |
| 85 | BrgMS777 | 3 | 3 | 3 | 2 | 2 | 3 | 3 | 2 | 2 | 2 | 0.57 |
| 86 | BrgMS309 | 2 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0.12 |
| 87 | BrgMS338 | 3 | 4 | 3 | 2 | 2 | 4 | 2 | 0 | 2 | 2 | 0.81 |
| 88 | BrgMS782 | 1 | 3 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 3 | 0.64 |
| 89 | BrgMS457 | 1 | 3 | 3 | 3 | 2 | 2 | 3 | 2 | 1 | 1 | 0.71 |
| 90 | BrgMS710 | 3 | 1 | 2 | 1 | 1 | 2 | 2 | 1 | 1 | 3 | 0.56 |
| 91 | BrgMS732 | 4 | 3 | 2 | 1 | 4 | 3 | 3 | 3 | 3 | 2 | 0.68 |
| 92 | BrgMS372 | 2 | 2 | 2 | 1 | 3 | 3 | 1 | 3 | 1 | 1 | 0.65 |
| 93 | BrgMS339 | 2 | 1 | 1 | 2 | 4 | 2 | 1 | 0 | 2 | 1 | 0.71 |
| 94 | BrgMS409 | 2 | 1 | 1 | 1 | 1 | 2 | 2 | 1 | 1 | 2 | 0.32 |
| 95 | BrgMS753 | 1 | 1 | 1 | 1 | 1 | 1 | 3 | 2 | 3 | 4 | 0.55 |
| 96 | BrgMS421 | 3 | 2 | 2 | 1 | 2 | 2 | 1 | 2 | 2 | 2 | 0.56 |
| 97 | BrgMS799 | 2 | 2 | 2 | 2 | 3 | 2 | 1 | 2 | 1 | 1 | 0.54 |
| 98 | BrgMS343 | 1 | 1 | 1 | 1 | 1 | 1 | 2 | 1 | 2 | 2 | 0.35 |
| 99 | BrgMS638 | 1 | 1 | 2 | 3 | 2 | 2 | 2 | 2 | 2 | 2 | 0.60 |
| 100 | BrgMS515 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 2 | 1 | 1 | 0.05 |
| 101 | BrgMS354 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | - |
| 102 | BrgMS777 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | - |
| 103 | BRMS-002 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 0.56 |
| 104 | BRMS-016 | 2 | 1 | 1 | 1 | 1 | 2 | 3 | 2 | 2 | 2 | 0.67 |
| 105 | BRMS-011 | 1 | 1 | 2 | 4 | 3 | 2 | 0 | 0 | 0 | 0 | 0.72 |
| 106 | BRMS-005 | 2 | 1 | 2 | 1 | 3 | 2 | 1 | 1 | 1 | 1 | 0.65 |
| 107 | BRMS-003 | 1 | 1 | 1 | 1 | 2 | 2 | 1 | 1 | 1 | 1 | 0.47 |
| 108 | BRMS-029 | 1 | 2 | 1 | 1 | 2 | 2 | 1 | 2 | 1 | 1 | 0.48 |
| 109 | BRMS-007 | 2 | 3 | 1 | 1 | 2 | 3 | 1 | 1 | 1 | 1 | 0.78 |
| 110 | BRMS-015 | 2 | 2 | 2 | 1 | 2 | 2 | 2 | 1 | 3 | 2 | 0.49 |
| 111 | BRMS-006 | 4 | 2 | 3 | 1 | 2 | 4 | 2 | 0 | 0 | 0 | 0.75 |
| 112 | BRMS-014 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | - |
| 113 | BRMS-033 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | - |
| C) B. napus-derived SSRs | ||||||||||||
| 114 | MB4 | 2 | 2 | 1 | 1 | 1 | 1 | 2 | 1 | 1 | 1 | 0.47 |
| 115 | MR52a | 2 | 1 | 2 | 1 | 1 | 3 | 2 | 1 | 2 | 2 | 0.53 |
| 116 | MR33 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | - |
| 117 | MR176 | 2 | 5 | 4 | 2 | 3 | 3 | 2 | 1 | 3 | 2 | 0.77 |
| 118 | MB5 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | - |
| D) B. oleracea-derived SSRs | ||||||||||||
| 119 | sORA43 | 1 | 3 | 2 | 4 | 3 | 2 | 2 | 1 | 1 | 3 | 0.68 |
| 120 | Ol09A01 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | - |
| 121 | Ol10B01 | 2 | 2 | 2 | 4 | 3 | 3 | 1 | 1 | 2 | 2 | 0.73 |
| 122 | Ol10B07 | 0 | 1 | 1 | 2 | 1 | 3 | 3 | 0 | 3 | 5 | 0.79 |
| 123 | Ol10B11 | 3 | 3 | 3 | 3 | 2 | 3 | 3 | 1 | 1 | 2 | 0.81 |
| 124 | sORA26 | 2 | 2 | 2 | 2 | 2 | 3 | 1 | 3 | 1 | 2 | 0.59 |