Table 1.
HisN paralogs in C. glutamicum
| HisN paralog (gene) | protein length [aa] | sequence similarity on protein level (identity, similarity, BlastP score) [%], [%] [bits] | annotation | HolPase activity | ||||
|---|---|---|---|---|---|---|---|---|
| HisN | Cg0911 | ImpA | SuhB | CysQ | ||||
| HisN (cg0910) |
260 | 100, 100 515 |
26, 41 78 |
25, 41 86 |
27, 42 82 |
24, 37 52 |
HolPase a | yes a, b |
| Cg0911 (cg0911) |
288 * | 26, 41 77 |
100, 100 585 |
22, 35 58 |
27, 41 89 |
21, 30 84 |
putative IMPase c or archaeal FBPase d |
yes (very low) b |
| ImpA (cg2298) | 275 | 25, 41 81 |
22, 35 74 |
100, 100 561 |
22, 32 80 |
15, 26 35 |
putative IMPase c or archaeal FBPase d |
not detected |
| SuhB (cg2090) | 280 | 27, 42 82 |
27, 41 89 |
22, 32 80 |
100, 100 567 |
23, 34 48 |
putative IMPase c or archaeal FBPase d |
not detected |
| CysQ (cg0967) | 252 | 24, 37 52 |
21, 30 59 |
15, 26 35 |
23, 34 48 |
100, 100 521 |
putative PAPSPase c, d | not detected |
* Based on RNAseq results, transcription of cg0911 starts 9 nt downstream of the currently annotated cg0911 CDS [5], resulting in a shorter Cg0911 protein (288 aa instead of 291 aa)
a [7]
b this study
c GenBank: BX927147.1 [21]
d GenBank: BA000036.3 [22]
PAPSPase = 3'-phosphoadenosine 5'-phosphosulfate (PAPS) 3'-phosphatase