Table 3.

List of potential HisN orthologs. Characteristic amino acid residues of HolPase motif 5 are underlined. HolPase activity was inferred from the ability of the corresponding gene to complement a C. glutamicum ΔhisN mutant

potential HisN ortholog	protein length [aa]	sequence similarity on protein level (identity, similarity, BlastP score) [%], [%] [bits]					HolPase motif 5	HolPase activity
		HisN	Cg0911	ImpA	SuhB	CysQ
Dietzia sp. strain Chol2 HisNDz	278	29, 47 105	25, 40 76	23, 33 69	26, 38 49	23, 35 46	yes RFGGDCYAY	yes
Zymomonas mobilis HisNZm	259	32, 48 101	22, 35 69	22, 36 58	26, 40 58	24, 37 62	yes LLGGDCYNY	yes
Actinoplanes utahensis HisNAu	266	55, 68 261	27, 40 76	26, 40 88	26, 40 71	26,32 56	yes RAYGDFYGY	yes
Actinoplanes utahensis HisN2Au	259	36, 51 119	24, 35 70	24, 39 61	27, 36 67	24, 37 58	no ------QPS	no