Table 4.

List of randomly chosen bacterial IMPase-like proteins that are most likely wrongly annotated with a focus on HolPases. All proteins have been compared to the five IMPase-like proteins in C. glutamicum and reannotated according to the detected motifs

Organism	GI-number	current annotation	protein with highest similarity in C. glutamicum	presence of expected motifs	similarity to HisNCg (BlastP score) [bits]	new functional assignment
Class 1
Pseudomonas pseudoalcaligenes	489546409	IMPase	HisNCg	yes	117	HisN
Rudaea cellulosilytica	648601632	IMPase	HisNCg	yes	108	HisN
Marinobacter psychrophilus	860341079	IMPase	HisNCg	yes	110	HisN
Rubrobacter radiotolerans	627778235	FBPase- or IMPase	HisNCg	yes	123	HisN
Class 2
Streptosporangium roseum	502655523	IMPase	HisNCg	no	120	-
Rubrobacter xylanophilus	499884516	IMPase	HisNCg	no	120	-
Class 3
Sediminibacterium salmoneum	739492005	HolPase	SuhBCg	yes	102	SuhB
Flavihumibacter solisilvae	743029970	HolPase	SuhBCg	yes	91	SuhB
Desulfatibacillum aliphaticivorans	654864062	HolPase	SuhBCg	yes	102	SuhB
Leptospirillum ferriphilum	738123182	HolPase	SuhBCg	yes	89	SuhB