Fig. 2.

Engineering of PapMV coat protein carrying the SrtA recognition motif. a Amino acid sequence of the PapMV CP C-terminus of the WT CP as compared to the engineered PapMV CP that harbours a linker of 5 amino acids (TSTTR) followed by the SrtA recongnition motif (LPETGG); a 6×His tag was inserted at the C-terminus of the PapMV coat protein (named PapMV-SrtA). b The length of the rod-shaped nanoparticles was assessed by dynamic light scattering (DLS). PapMV-SrtA (40 nm) was showed to be slightly smaller than the WT PapMV (54 nm). c Transmission electron microscopy (TEM) of WT PapMV (left) and PapMV-SrtA (right) nanoparticles shows the rod shape of the nanoparticles