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. 2017 Jul 18;18:539. doi: 10.1186/s12864-017-3902-4

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — 2D–HSQC-NMR spectra of pith and xylem tissues. a, c Aliphatic/aldehyde and b, d aromatic regions of ¹H–¹³C (HSQC) spectra from cell wall gel samples in DMSO-d₆:pyridine-d₅ (4:1). a, b: Pith tissue. c, d: Xylem tissue. Levels of A, B and C in pith (A) were not quantified because of co-located polysaccharides with Aα, however the spectra allow the relative estimation. NMR lignin correlations are coloured to match the structures responsible. ABL refers to total lignin content measured as acetyl bromide lignin