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. 2017 Jul 18;17:490. doi: 10.1186/s12885-017-3478-z

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — 3D culture in alginate favors expression of collagen type II by chondrosarcomas. Chondrosarcomas were cultured in monolayer, or embedded in alginate for 1, 2 or 3 weeks. Then, alginate was dissolved and RNA extracted. Collagen type II and type I, and COMP mRNA levels were assayed by real-time RT-PCR after normalization to RPL13 signal. Values are the mean ± SEM of triplicate experiments