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. 2016 Jun 9;2:16019. doi: 10.1038/npjmgrav.2016.19

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Copyright © 2016 Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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GFAP and AQP4 staining in the brain. Representative micrographs of brain sections were evaluated for astrocytes by immunostaining with anti-4-hydroxynonenal (GFAP) and AQP4 antibodies on samples collected at 9 months after exposure. AQP4-positive staining is identified by green fluorescence, GFAP with red, and the cell nuclei with blue (DAPI). Increased AQP4 staining was seen in the combination group compared with controls. Magnification is x400. DAPI, 4ʹ, 6-diaminodino-2-phenylindole.