Figure 3.

Expression of steroidogenic enzyme proteins by the HFA during GW9–12. (A). Protein levels were analyzed by Wes-automated Western blotting and representative blots are shown to the left. To the right, band densities are expressed as a percentage of the corresponding density of GW9. The values shown are means ± s.e. for three (GW9–11) and two (GW12) HFA isolated from individual fetuses. *P < 0.05 compared to GW9. (B) Expression of androgen-metabolizing enzymes in the HFA at GW10 identified by immunohistochemistry. Inserts show the absence of immunostaining in the presence of non-immune serum instead of primary antibody. Scale bar: 100 μm.