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. Author manuscript; available in PMC: 2018 May 30.
Published in final edited form as: Analyst. 2017 May 30;142(11):1847–1866. doi: 10.1039/c7an00198c

Figure 12.

Figure 12

Base peak electropherograms for a two-antibody mixture (mouse anti-human IgG4 Fc-UNLB and human/mouse/rat activin A beta A subunit antibody), including migration times for each heavy chain and light chain. Triplicate CZE–ESI–MS analysis of a two-antibody mixture. 50 cm long, 30 μm I.D. capillary was used for separation. About 30 nL liquid aliquot was injected for each analysis. Sheath liquid: 0.5% FA, 10% methanol. Voltages: 23.7 kV/1.2 kV. LTQ-XL mass spectrometer was used for detection. NL is the normalization level. (Modified from reference168 with permission)