Figure 4.

Electropherograms with (red trace) or without (black trace) cross-linking of protein complexes of (A) Hsp70–488–Bag3, (B) Hsp90-488 dimer, and (C) FITC-lysozyme-antilysozyme. Separation was performed with (A,B) dextran gel and (C) free solution with pH 10, 10 mM borate buffer. Cross-linking was with 1% formaldehyde for 10 min in HEPES buffer. (Reprinted from reference⁶¹ with permission)