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. Author manuscript; available in PMC: 2018 Jul 1.
Published in final edited form as: Genesis. 2017 Jun 2;55(7):10.1002/dvg.23039. doi: 10.1002/dvg.23039

Figure 2. Generation of Golga5-deficient mice using CRISPR/Cas9-mediated genome editing.

Figure 2

(A) Top row shows schematic presentation of the genomic organization of part of the Golga5 gene. Boxes marked by E1 to E6 represent exons. Two red arrows mark the target sites of two guide RNAs (sgRNAs) designed to targeted exon-3 and exon-4, respectively. The sgRNA target sequences are shown in orange (E3-sgRNA) or red (E4-sgRNA) font with the PAM sequences shown in green. The frame-shift indel mutations found in founder mice are listed in comparison to the wildtype sequences corresponding to the two target sites. (B, C) Frontal sections through the Meckel’s cartilage region of E13.5 Golga5E4-13bp/+ heterozygous (B) and Golga5E4-13bp/E4-13bp homozygous (C) embryos showing co-immunofluorescent staining for GM130 (red) and GOLGA5 (green) proteins. DAPI counterstaining of nuclei was shown in blue. (D) Western blot detection of GOLGA5 and β-actin proteins in the control and Golga5E4-13bp/E4-13bp or Golga5E3-4del/E3-4del tissue lysates.