Figure 1.
WJ-MSC gene expression of immunomodulatory molecules. Cells were seeded at a density of 4000 cells per cm2, and samples were collected at 3 different passages. After reaching 80% of confluence, cells were lysed, total RNA extracted, and real-time PCR performed. IL-1α, IL-1β, IL-6, IL-8, IL-10, IL-11, HGF, TGF-β1, IDO, and LIF gene expression and protein secretion from WJ-MSC1, WJ-MSC2, and WJ-MSC3. (a) Gene expression and (b) protein secretion. Gene expression was normalized by housekeeping gene GAPDH and expressed as fold change compared to the control—passage 5. Experiments were performed in triplicate. Results are represented as mean ± SD (n = 3).