Figure 2.

WJ-MSC inhibit CD3⁺ T cell proliferation. WJ-MSC were seeded, after 24 hours. PBMC were added and stimulated with PHA for 3 days in the presence of MSC; the ratio used was 1 : 10 (WJ-MSC : PBMC). The WJ-MSC passages used in experiments were either P5 or P6. T cells were collected and stained with anti-CD3 antibody and proliferation measured by flow cytometry using the Click It Kit. The percentage of inhibition (Inhib) was calculated using the percentage of proliferation of PBMC + PHA + WJ-MSC compared to the control PBMC + PHA. Experiments were performed in triplicate. Results are represented by mean ± SD. Statistically significant differences are shown as ^∗∗∗p < 0.0001 (n = 3).