Figure 3.

WJ-MSC inhibit CD3⁺ CD4⁺ and CD3⁺ CD8⁺ T cell proliferation. WJ-MSC were seeded, after 24 hours. PBMC was added and stimulated with PHA for 3 days in the presence of WJ-MSC; the ratio used was 1 : 10 (WJ-MSC : PBMC). The WJ-MSC passages used in experiments were either P5 or P6. T cells were collected, stained with anti-CD3, anti-CD4, and anti-CD8 antibodies, and proliferation was measured by flow cytometry using the Click It Kit. (a) % proliferation of CD3⁺CD4⁺Click It⁺ T cell and (b) % proliferation of CD3⁺CD8⁺Click It⁺ T cell. The percentage of inhibition (Inhib) was calculated using the percentage of proliferation of PBMC + PHA + WJ-MSC compared to the control PBMC + PHA. Experiments were performed in triplicate. Results are represented by mean ± SD. Statistically significant differences are shown as ^∗∗∗p < 0.0001 (n = 3).